LED Array System
LED Array System
Optogenetics became explosively popular
for controlling animal behaviour in-vivo,
however, recently this technology was
applied for in-vitro cells or tissues for
controlling gene expression.
For this purpose, long-term and
time-controlled light stimulation in a
culture incubator is required..
This full waterproof LED array fulfills
all the requirements for the in-vitro
optogenetics experiments
LED Array
model: LEDA-x
x: color code, see the table at bottom-left
LED Array Driver
model: LAD-1
Fits perfectly for multi-well plate
6 well
Upper view with 96 well plate
It is designed for any commercial, standard-sized multi-well plates so can be used together with e.g. 6, 12, 24, 48 and 96 well plates.
Especially it perfectly fits for standard-sized 96 well plate because each LED element comes just under each well.
Trigger Input
By the mode switch of LAD-1 LED Array Driver you can
choose constant mode or trigger mode. In trigger mode,
the Trg In BNC on the back panel is used for receiving
trigger TTL pulses from a stimulator so that it enables
time-controlled pulsed stimulation in-vitro.
Many Color Options
| Model | Descriptions |
|---|---|
| LAD-1 | LED Array Driver |
| LEDA-365 | LED Array, 365nm |
| LEDA-V | LED Array, 400nm |
| LEDA-420 | LED Array, 420nm |
| LEDA-450 | LED Array, 450nm |
| LEDA-B | LED Array, 470nm |
| LEDA-500 | LED Array, 500nm |
| LEDA-G | LED Array, 530nm |
| LEDA-Y | LED Array, 590nm |
| LEDA-R | LED Array, 630nm |
| LEDA-670 | LED Array, 670nm |
| LEDA-740 | LED Array, 740nm |
| LEDA-850 | LED Array, 850nm |
| LEDA-I | LED Array, 950nm |
| STOmk-2 | Stimulator for Optogenetics |
* For other colors, please contact us.
4 Section LED Array System
model: LEDA4-x
model: LAD-4
LED4-x has 4 independently controllable sections each having 24 LED.
LAD-4 is independent 4ch LED array driver designed for controlling LEDA4-x. It has 4 trigger input BNC on the back panel for independent control of each section in LEDA4.
4 Channel Stimulator
MODEL: STO-4
STO-4 is 4ch version of our STOmk-2 stimulator for optogenetics.
STO-4 is 4 channel constant voltage pulse generator, and each channel can be controlled independently. The output is 0 ~ 5V analog voltage, which is suitable for controlling analog-voltage compatible light sources, e. g. our LAD series LED array system. Especially, it is ideal for controlling 4 section LED array (LEDA4-x and LAD-4).
2 Color LED Array System
Model: LEDA2-x/x
* x: color code. see the table above for color options.
In the LEDA2 two color LED array system, you can pick any two colors from the color options in the table above. The two LEDs are alternately arranged and the boarder comes to the center of each well in 96 well plate. It is suitable for excitatory and inhibitory control in optogenetic experiment, expression control of two different genes, etc. For independent control of each color, 2x LAD-1 are required. 1x STOmk-2 can control both colors because it has two independent channels.
6 section LED Array System
* x: color code. see the table above for color options.
LEDA6 has 6 independently controllable sections each having 16 LEDs. It is suitable for 6 well plate experiment enabling independent light power and stimulation timing control.
LAD-6 is independent 6ch LED array driver designed for controlling LEDA6. Unlike LAD-4, it does not have trigger input BNC on the back panel, instead it is connected to PC via USB cable and controlled by LAD6 software.
Optogenetic Place Aversion System
In 2014, Lyer et al. published a work using a new pain test model, combining optogenetics technique and conventional place aversion test:
Virally mediated optogenetic excitation and inhibition of pain in freely moving nontransgenic mice. Nat Biotechnol In this Optogenetic Place Aversion (=OPA) test, the result does not depend on experimenters skill unlike the traditional Von-Frey test. In addition, you can evaluate pain sensory neuron-specifically (e.g. A-beta, A-delta, C fiber, etc.), by expressing ChR2 on specific neurons. Our OPA system uses
480 pieces of high power LED for each array so that enough light can reaches sensory neuron under animal's skin, thus enables non-invasive and objective test.
LED Array for OPA
- 480 pieces LED per array
- Dedicated high-power LED Array driver (LAD-1-OPA)
- Minimized heat generation: the air layer between LED and top plate blocks heat. Most heat goes to alminum body under the LED array, maximizing heat dissipation.
- Blue (470nm) and Yellow (590nm) by default. Other colors are possible on request.
model: OPA-SYSTEM, OPA-BOX, LAD-1-OPA, LEDA-B-OPA, LEDA-Y-OPA
Data Recording
Using a camera from side or top of the test box, animal position is continuously tracked and recorded. Total time in each side, the time ratio between blue and yellow zone can be caluculated.
These are main parameters which can be used as quantified pain evaluation index.
Contents
OPA-SYSTEM Optogenetic Place Aversion System (includes:)
- OPA-BOX OPA test box
- LAD-1-OPA LED Arrray Driver for OPA x2
- LEDA-B-OPA LED Array for OPA, Blue
- LEDA-Y-OPA LED Array for OPA, Yellow
- VTS-4 Video Tracking Stimulator