LED Array System

LED Array System

LED Array System

Optogenetics became explosively popular
for controlling animal behaviour in-vivo,
however, recently this technology was
applied for in-vitro cells or tissues for
controlling gene expression.
For this purpose, long-term and
time-controlled light stimulation in a
culture incubator is required..
This full waterproof LED array fulfills
all the requirements for the in-vitro
optogenetics experiments
#
LED Array

model: LEDA-x

x: coler code, see bottom-left of this page

LAD-1
LED Array Driver

model: LAD-1

Fits perfectly for multi-well plate

#

6 well

#

Upper view with 96 well plate

It’s designed for any of commercial multi-well plates so can be used together with e.g. 6, 12, 24, 48 and 96 well plates.
Especially it’s perfectly fits for 96 well plate because each LED element comes just under each well.

Trigger input

#
By the mode switch of LAD-1 LED Array Driver you can
choose constant mode or trigger mode. In trigger mode,
the Trg In BNC on the back panel is used for receiving
trigger TTL pulses from a stimulator so that it enables
time-controlled pulsed stimulation in-vitro.

Many color options

#

470nm

#

590nm

#

530nm

#

630nm

#
Two color LED Array

model:LEDA2-BY


Color code

V: 400nm / B: 470nm / G: 530nm / Y: 590nm / R: 630nm / I: 940nm

* contact us for other colors

4 Section LED Array System

#

model: LEDA4-x

#

model: LAD-4

LED4-x has 4 independently controllable sections each having 24 LED.

LAD-4 is independent 4ch LED array driver designed for controlling LEDA4-x.

4 Channel Stimulator

#

MODEL: STO-4

STO-4 is 4ch version of our STOmk-2 stimulator for optogenetics.

STO-4 is 4 channel constant voltage pulse generator, and each channel can be controlled independently. The output is 0 ~ 5V analog voltage, which is suitable for controlling analog-voltage compatible light sources, e. g. our LAD series LED array system. Especially, it is ideal for controlling 4 section LED array (LEDA4-x and LAD-4).

Optogenetic Place Aversion System

#

In 2014, Lyer et al. published a work using a new pain test model, combining optogenetics technique and conventional place aversion test:

Virally mediated optogenetic excitation and inhibition of pain in freely moving nontransgenic mice. Nat Biotechnol In this Optogenetic Place Aversion (=OPA) test, the result does not depend on experimenters skill unlike the traditional Von-Frey test. In addition, you can evaluate pain sensory neuron-specifically (e.g. A-beta, A-delta, C fiber, etc.), by expressing ChR2 on specific neurons. Our OPA system uses
480 pieces of high power LED for each array so that enough light can reaches sensory neuron under animals’ skin, thus enables non-invasive and objective test.
#

LED Array for OPA

  • 480 pieces LED per array
  • Dedicated high-power LED Array driver (LAD-1-OPA)
  • Minimized heat generation: the “air layer” between LED and top plate blocks heat. Most heat goes to alminum body under the LED array, maximizing heat dissipation.
  • Blue (470nm) and Yellow (590nm) by default. Other colors are possible on request.
#
#

model: OPA-SYSTEM, OPA-BOX, LAD-1-OPA, LEDA-B-OPA, LEDA-Y-OPA

Data Recording

Using a camera from side or top of the test box, animal position is continuously tracked and recorded. Total time in each side, the time ratio between blue and yellow zone can be caluculated.
These are main parameters which can be used as quantified pain evaluation index.

Contents

OPA-SYSTEM Optogenetic Place Aversion System (includes:)
  • OPA-BOX OPA test box
  • LAD-1-OPA LED Arrray Driver for OPA x2
  • LEDA-B-OPA LED Array for OPA, Blue
  • LEDA-Y-OPA LED Array for OPA, Yellow
  • VTS-4 Video Tracking Stimulator