TeleFipho
Fiber photometry is a powerful technique to detect calcium signal from specific neuron in awake animals using calcium indicator protein represented by GCaMP. In freely moving condition, the long optic fiber attached to the head of the animal can interfere with experimental setup, therefore can be a limitation factor of your project.
Our innovative new product, TeleFipho, includes all required components for fiber photometry - optic fiber, filter cube, light source, photo detector - and also wireless transmission circuit, in the very smal 3g body. TeleFipho definitely will not block free behavior of your animals, enabling novel experimental approach using fiber photometry.
Stress induced GCaMP signal from orexin neuron in mouse hypothalamus. By courtesy of Dr. Daisuke Ono in Akihiro Yamanaka Lab, Nagoya University.
Activity synchronized GCaMP signal in mouse dorsal striatum. LPF 5Hz is applied. By courtesy of Dr. Keitaro Yoshida in Kenji Tanaka Lab, Keio Univeristy.
Simple setup - headstage, receiver and software
Small headstage, suitable for mice
Features
- World first commercial wireless fiber photometry
- Small headstage / good for mice, rats, marmosets, etc.
- Standard 2.5mm ferrule cannula
- Rechargable by a dedicated charger
- Adjustable excitation LED power
- Adjustable signal offset
- For GCaMP or GFP-like indicators
| Headstage weight | 3g |
| Headstage size | 15 x 15 x 22 mm |
| Excitation wavelength | LED peak 470nm, Filter band 445～490nm |
| Emission wavelength | Filter band 500～550nm |
| Excitation power | 10～300µW＠400µm Fiber end (Adjustable) |
| Sampling rate | 100Hz |
| AD resolution | 16bit |
| Photo sensor | Photo diode |
| Gain | 1010 V/A |
| Battery life | 2hours＠Excitation power 30µW |
| Transmission band | 2.4GHz |
| Transmission distance | 2m |
| Power | Battery powered, rechargeable |
| Receiver I/O | 1x Photometry analog out, 1x Geneal purpose analog In (0~5V) |
| PC Interface | USB / TeleFipho software (for Windows10) |
| Cannula | core: 400µm/NA0.39, Cladding: 425µm, Ferrule: 2.5mm core: 200µm/NA0.39, Cladding: 225µm, Ferrule: 2.5mm core: 600µm/NA0.39, Cladding: 630µm, Ferrule: 2.5mm |
TeleFipho Standard Set
TeleFipho Standard Set consists of the following items：
- TeleFiT 1x TeleFipho Transmitter Headstage
- TeleFiR 1x TeleFipho Receiver
- TeleFiCharger 1x TeleFipho Charger
- TeleFiC-l-d (※) 3x TeleFipho Cannula
- TeleFiTool 1x Insertion Tool for TeleFipho
- TeleFiDummy 1x TeleFipho Dummy Headstage
- 1x TeleFipho software installer
- 1x pMAT Converter software installer
(※) Please specify cannula length and fiber diameters. 5mm length & 400µm core cannula will come without specifying.
TeleFiC-l-d l: length in mm (resolution 0.1mm) / d: core diameter in µm (400 or 200)
e.g. Cannula length 3.5mm & core 400µm: TeleFiC-3.5-400
Transmitter Headstage
Receiver
Charger
Cannula
Insertion Tool
Dummy Headstage
Stand alone / External recorder
TeleFipho receiver can be directly connected to PC via USB for fiber photometry data recording in TeleFipho software. TeleFipho software is good enough for simple raw data / low pass filtered data recording.
Data can be directly read from Matlab / Octave, or can be exported to ASCII format.
We recommend the open-source pMAT software for delta F/F and PETH and analysis, developed by Dr. David Barker in Rutgers University. For more details please see the following support page:
Analyze TeleFipho Data in pMAT software
For more complicated online analysis and / or parallel recording of other physiological data, you can access photometry data as analog signal via BNC on the front panel of TeleFipho receiver so that you can connect external data recording system (e.g. PowerLab from ADI).
| Model | Description |
|---|---|
| TeleFipho-set | TeleFipho Standard Set |
| TeleFiT | TeleFipho Transmitter Headstage |
| TeleFiR | TeleFipho Receiver |
| TeleFiCharger | TeleFipho Charger |
| TeleFiC-l-d | TeleFipho Cannula |
| TeleFiTool | Insertion Tool for TeleFipho |
| TeleFiDummy | TeleFipho Dummy Headstage |